2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (2106):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0711
    Indocyanine green 3599-32-4 ≥98.0%
    Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Indocyanine green
  • HY-D0717
    DAF-FM DA 254109-22-3 ≥98.0%
    DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).
    DAF-FM DA
  • HY-B1046
    Clofazimine 2030-63-9 99.20%
    Clofazimine is an orally-active anti-mycobacterial agent with a wide range of anti-mycobacterial activity including leprosy and tuberculosis. Clofazimine exerts anti-inflammatory activities and anti-tumor activities by interfering DNA replication and inhibiting IL2 (IC50 = 1.10 ± 0.26 μM, Jurkat T) production. Clofazimine can be used in mycobacterial and cancer research.
    Clofazimine
  • HY-14536
    Methylene Blue 61-73-4 ≥98.0%
    Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.
    Methylene Blue
  • HY-19948
    Leucomethylene blue mesylate 1236208-20-0 ≥98.0%
    Leucomethylene blue (TRx0237) mesylate, an orally active second-generation tau protein aggregation inhibitor (Ki of 0.12 μM), could be used for the study of Alzheimer's Disease. Leucomethylene blue mesylate is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes.
    Leucomethylene blue mesylate
  • HY-111377
    Amine-PEG3-Biotin 359860-27-8 98.41%
    Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.
    Amine-PEG3-Biotin
  • HY-D0006
    Bathophenanthroline 1662-01-7
    Bathophenanthroline is an agent used for the measurement of iron concentrations in aqueous , serum and urine samples by colorimetry.
    Bathophenanthroline
  • HY-D0516
    Solvent Blue 35 17354-14-2 98.44%
    Solvent Blue 35 (Sudan Blue II; Oil Blue 35) is a dye used for colouring alcoholic and hydrocarbon based solvents. It is used for staining triglycerides in animal tissues.
    Solvent Blue 35
  • HY-15907
    BBD 18378-20-6 ≥98.0%
    BBD (NSC240867) is a biochemical reagent/chromogenic reagent.
    BBD
  • HY-112276
    Beryllon II 51550-25-5 ≥98.0%
    Beryllon II is a widely used chromogenic reagent that is used to determine many elements, such as Mo, Mg and Co, and also used for the determination of proteins.
    Beryllon II
  • HY-15905
    ALPS 82611-85-6 99.41%
    ALPS(N-Ethyl-N-sulfopropylaniline sodium salt) is a bio-chemical reagents/chromogenic reagent.
    ALPS
  • HY-15935
    X-Gluc Dicyclohexylamine 18656-96-7 99.48%
    X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system).
    X-Gluc Dicyclohexylamine
  • HY-D0828
    3,6-Dichlorotrimellitic acid 137071-78-4 99.31%
    3,6-Dichlorotrimellitic acid is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.
    3,6-Dichlorotrimellitic acid
  • HY-D1505
    4-(N,N-Dimethylamino)azobenzene-4'-isothiocyanate 7612-98-8
    4-(N,N-Dimethylamino)azobenzene-4'-isothiocyanate is a chromophoric, hydrophobic reagent for probing membrane-buried segments of intrinsic proteins.
    4-(N,N-Dimethylamino)azobenzene-4'-isothiocyanate
  • HY-15903
    ADOS 82692-96-4
    ADOS(82692-96-4) is a biochemical reagent/chromogenic reagent.
    ADOS